How do I…

  • genotype a mutation?

 

  • determine where a gene is expressed?

 

  • determine when a gene is expressed?

 

  • find homologous C. elegans genes to a human gene?
    • Web search for the human gene, e.g. CACNA1A – information on function, location, etc.
    • Web search for human protein sequence: click on UniProt link or go directly to uniprot.org
    • Scroll down to “Sequences” and choose “FASTA” (Sequence data in FASTA format).  Copy the text sequence.
    • Go to wormbase BLAST and paste the human protein sequence. Select Query Type: protein and OK.
    • Locate genes with highest alignment, greatest Score (bits), and an E-value of  0.0
    • In this example, the gene unc-2 has:
      • Score 901 bits, E-value = 0, Identities = 439/767 (57%), Positives = 558/767 (72%)
      • Click [Gene Summary] to go to the WormBase gene, unc-2 to verify that the reported worm gene function matches the human gene function
      • Click [Alignment] to view exact amino acid matches and mismatches